Obesity and Cholesterol
The full text for this paper may be found in the Journal of Nutritional
Biochemistry volume 7 pp 106-112 1996
EFFECT OF 22-5 -TAUROCHOLENIC ACID AND DIETARY FAT ON
HEPATIC CHOLESTEROL AND FATTY ACID IN HYPERGLYCEMIC-OBESE MICE
G. Richard Jansen
Department of Food Science and Human Nutrition
Colorado State University
Fort Collins, Colorado, U.S.A. 80523
Our Previous work has demonstrated that hyperglycemic obese mice (obob)
fed a 1% corn oil diet accumulated cholesterol and fat in the liver when
compared to non- obese littermates. The present experiment was carried
out to test the hypothesis that feeding the bile acid analog
22-5 -taurocholenic acid would correct this metabolic defect. Liver
total fatty acid was greatly elevated in obob mice fed 1% corn oil compared
to non-obese controls (21.04 ± 1.23% vs 4.41 ± 0.27%). Incorporation
of [U-14] glucose into liver fatty acid was inhibited by taurocholenic
acid in both obese and non-obese mice fed 1% corn oil. Taurocholenic
acid reduced liver fatty acid in obob mice fed 1% corn oil to the level
seen in non-obese mice (3.07 ± 0.22% vs 4.41 ± 0.27%).
Cholesterol greatly accumulated in the livers of obob mice fed 1% corn
oil compared to non-obese mice (0.97 ± 0.08% vs 0.28 ± 0.02%)
and was reduced to normal by feeding taurocholenic acid (0.27 ±
0.05% vs 0.28 ± 0.01%) in spite of greatly elevating rates of incorporation
of [U-14C]glucose into liver cholesterol. Taurocholenic acid significantly
reduced liver fat in obese mice fed 40% lard (11.62 ± 1.41% vs 5.87
± 1.03%) but had no significant effect on the percentage of liver
cholesterol (0.68 ± 0.05% vs 0.56 ± 0.04%). It is suggested
that the elevation of liver cholesterol in obobmice fed 1% corn oil comes
about through a defect in cholesterol removal from the liver, a defect
corrected by taurocholenic acid via increasing the excretion of cholesterol
and bile acids in the bile by an as yet unknown mechanism. The cholesterol
accumulating in obob mice fed 40% lard occurs by a different mechanism
than when these mice are fed 1% corn oil since it is unaffected by taurocholenic
acid.
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