Department of Biochemistry & Molecular Biology, Colorado State University,
Fort Collins, CO 80523, USA.
Tel: (970) 4910436 (work); Fax: (970) 491-0494
E-mail: Narasimha.Sreerama@ColoState.Edu
1983 - 1988 Ph.D. Indian Institute of Science, Bangalore, India
1981 - 1983 M.Sc. Mysore University, Mysore, India
1978 - 1981 B.Sc. Mysore University, Mysore, India
1988 - 1989 Research Associate, Molecular Biophysics Unit, Indian Institute of Science., Bangalore, India
1989 - 1992 Post-doctoral Fellow, Department of Biochemistry & Molecular Biology, Colorado State University, Fort Collins, CO
1992 - 1997 Research Associate, Department of Biochemistry & Molecular Biology, Colorado State University, Fort Collins, CO
1998 - 2002 Research Assistant Professor, Department of Biochemistry & Molecular Biology, Colorado State University, Fort Collins, CO
2002 - Present Research Associate Professor, Department of Biochemistry & Molecular Biology, Colorado State University, Fort Collins, CO
1982 - 1983 Merit Scholarship, Mysore University, India
1983 - 1985 Junior Research Fellowship, Indian Institute of Science., India
1985 - 1988 University Grants Commission Scholar, India
1990 - Present American Chemical Society
1991 - Present Biophysical Society
1992 - 1995 Guest Lecturer in the course BC511 - "Macromolecular Structure & Function"
2000 - 2001 Guest Lecturer, BC513 - "Enzymology"
2001 - 2002 Guest Lecturer, BC611 - "Structural Biology II"
2002 - 2003 Guest Lecturer, BC611 - "Structural Biology II"
Computational: Performed ab initio and semi-empirical MO calculations; Performed crystal field calculations on nucleic acid bases; Developed software to compute electron density and rotational strengths; Developed methods to perform protein and nucleic acid CD calculations; Performed MD simulations.
Experimental: Performed protein-folding studies on T7 RNA polymerase using CD and fluorescence spectroscopy.
Molecular Modeling: Performed molecular dynamics simulations of myoglobin and protein secondary structures; Analyzed water structure from MD trajectories; Performed CD calculations of Ff gene-5-protein and BPTI mutants.
Computer Software: Developed software for the analysis of protein CD spectra (SELCON and CDPro); Developed software for computing protein and nucleic acid CD spectra; Developed algorithm for identifying protein secondary structure; Familiar with GROMOS, AMBER, INSIGHT and GAUSSIAN software packages; Familiar with FORTRAN, Pascal, and Unix.
Protein Structure Analysis: Developed algorithm for identifying poly(Pro)II conformation in protein structures; Developed methods to estimate protein secondary structures from CD spectra; Developed algorithm for predicting the number of a-helical and b-sheet segments from CD; Performed structural analysis of bI and bII proteins; Developed methods for analyzing membrane protein CD.
Bioinformatics: Developing methods to combine experimentally derived secondary structure information with sequence-based protein structure prediction algorithms.
Professional Service: Refereed articles for scientific journals; Written book chapters and review articles.